Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4886

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Intracellular Trafficking Studies Using Gold-Encapsulated Gelatin Nanoparticles

Sushma Kommareddy and Mansoor M. Amiji

This protocol was adapted from "Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles," Chapter 52, in Gene Transfer: Delivery and Expression of DNA and RNA (eds. Friedmann and Rossi). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2007.


INTRODUCTION

Trafficking of nanoparticles in cells is best carried out by encapsulation of electron-dense material (e.g., gold) in the nanoparticles and by visualizing them in the cell by transmission electron microscopy (TEM). This protocol describes the preparation of gold particles, their encapsulation in protein nanoparticles, culture conditions, and sample preparation for TEM.


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Protein Nanospheres for Gene Delivery
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