Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4917

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Misquitta, L.
Right arrow Articles by Paterson, B. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Misquitta, L.
Right arrow Articles by Paterson, B. M.
Related Collections
Right arrow RNA Interference (RNAi)/siRNA
Right arrow Cell Biology, general
Right arrow Preparation of Macromolecules and Introduction into Cells
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Drosophila
Right arrow Molecular Biology, general
Right arrow Transgenic Technology, general
Right arrow Drosophila Transgenics
Right arrow Developmental Biology
Right arrow DNA Delivery/Gene Transfer
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Non-Viral Methods
Right arrowRelated Article
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Collection of Drosophila Embryos for RNA Interference (RNAi)

Leonie Misquitta, Qin Wei, and Bruce M. Paterson

This protocol was adapted from "Targeted Disruption of Gene Function in Drosophila by RNA Interference," Chapter 19, in Drosophila Protocols (eds. Sullivan et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000. Please note that this version of the protocol is a 2007 revision.


INTRODUCTION

RNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a method for collection of Drosophila embryos for RNA interference (RNAi) experiments. The embryos are collected in a simple, homemade apparatus, arrayed on prepared glass slides, and readied for injection. It is important to keep the embryos moist and oxygenated. Work expeditiously, because embryos should be injected within 30-60 min after collection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

Drosophila RNA Interference (RNAi) Using a Gal-4 Inducible Transgene Vector
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols 2008: 51. [Abstract] [Full Text]

Related Protocols

Preparation of Double-Stranded RNA for Drosophila RNA Interference (RNAi)
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols 2008: 4916. [Abstract] [Full Text]

Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
Leonie Misquitta, Qin Wei, and Bruce M. Paterson
CSH Protocols 2008: 4918. [Abstract] [Full Text]