Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4933

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Scanning Electron Microscopy (SEM) of Plant Tissues

Kirsten Bomblies, Vipula Shukla, and Charles Graham

This protocol was adapted from "How to Analyze a Mutant Phenotypically," Chapter 4, in Arabidopsis (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

Scanning electron microscopy (SEM) is used to produce detailed images of surface structures. This article details the steps required to prepare a sample for SEM. In brief, the sample is fixed, dehydrated, subjected to critical-point drying, mounted, and coated with an electrically conducting material such as gold or palladium.


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