Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5029

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Southall, T. D.
Right arrow Articles by Brand, A. H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Southall, T. D.
Right arrow Articles by Brand, A. H.
Related Collections
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Drosophila
Right arrow Molecular Biology, general
Right arrow Analysis of Gene Expression
Right arrow Analysis of Gene Expression, general
Right arrow Expression of Cloned Genes
Right arrow Vectors
Right arrow Transgenic Technology, general
Right arrow Drosophila Transgenics
Right arrow Developmental Biology
Right arrow DNA Delivery/Gene Transfer
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Non-Viral Methods
Right arrowRelated Articles
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila

Tony D. Southall and Andrea H. Brand1

The Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom

1Corresponding author (ahb{at}mole.bio.cam.ac.uk)


INTRODUCTION

The GAL4 system is a method for ectopic gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. This protocol describes the generation of driver and reporter lines for use with the GAL4 system in Drosophila. A promoter-GAL4 fusion is constructed using a P-element transformable vector, and a GAL4-responsive target gene is created via generation of an upstream activation sequence (UAS)-reporter construct. An alternative strategy for integration using the phiC31 system is also provided. Transformant lines are generated using standard procedures for microinjection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Articles

The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
Tony D. Southall, David A. Elliott, and Andrea H. Brand
CSH Protocols 2008: 49. [Abstract] [Full Text]

Quantitative Microinjection of Drosophila Embryos: General Strategy
Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague
CSH Protocols 2007: 5. [Abstract] [Full Text]

Related Protocols

Directional Cloning into Plasmid Vectors
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 3919. [Extract] [Full Text]

Collection, Dechorionation, and Preparation of Drosophila Embryos for Quantitative Microinjection
Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague
CSH Protocols 2007: 4717. [Abstract] [Full Text]

Quantitative Microinjection of Drosophila Embryos
Daniel P. Kiehart, Janice M. Crawford, and Ruth A. Montague
CSH Protocols 2007: 4718. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
T. D. Southall, D. A. Elliott, and A. H. Brand
The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila
CSH Protocols, July 1, 2008; 2008(8): pdb.top49 - pdb.top49.
[Abstract] [Full Text]