Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4982

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Wees, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by van Wees, S.
Related Collections
Right arrow Plant Cell Culture
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Visualization, general
Right arrow Genetics, general
Right arrow Imaging/Microscopy, general
Right arrow Labeling for Imaging
Right arrow Laboratory Organisms, general
Right arrow Plant Biology, general
Right arrow Analysis of Gene Function in Plants
Right arrow Arabidopsis
Right arrow Phenotypic Analysis in Plants
Right arrow Transgenic Technology, general
Right arrow Phenotypic Analysis
Right arrow Transgenic Plants
Right arrow Cell Imaging
Right arrow Plant
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
BSN globe

protocolProtocol

Phenotypic Analysis of Arabidopsis Mutants: Trypan Blue Stain for Fungi, Oomycetes, and Dead Plant Cells

Saskia van Wees

This protocol was adapted from "How to Analyze a Mutant Phenotypically," Chapter 4, in Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

Trypan blue stains vasculature, dead plant cells, and fungal and oomycete hyphae. It is useful for assessing the extent of colonization of tissue, and for detecting microlesions present in certain lesion-mimic mutants. Trypan blue staining requires chloral hydrate for destaining, which is inconvenient, because it is a controlled substance. The chloral hydrate can be replaced with 1:2 lactophenol:ethanol, but the background staining will be higher than it is when chloral hydrate is used.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
D. Weigel and J. Glazebrook
Phenotypic Analysis of Arabidopsis Mutants: Bacterial Pathogens
CSH Protocols, July 1, 2009; 2009(7): pdb.prot4983 - pdb.prot4983.
[Abstract] [Full Text]