Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5036

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Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Probe Synthesis

Cecilia Moens

Division of Basic Science, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA

Corresponding author (cmoens{at}fhcrc.org)


INTRODUCTION

This protocol describes methods for synthesizing digoxigenin- and fluorescein-labeled RNA probes from linearized plasmids or PCR products for use in whole-mount RNA in situ hybridization on zebrafish embryos.


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