Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot5038

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Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Mounting

Cecilia Moens

Division of Basic Science, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA

Corresponding author (cmoens{at}fhcrc.org)


INTRODUCTION

This protocol describes the mounting of zebrafish embryos for observation after whole-mount in situ hybridization. Embryos can be mounted in glycerol or dehydrated and mounted in Permount, with or without the yolk. Glycerol causes less shrinkage, but is not as effective a clearing agent. Embryos mounted on the yolk should be stored in the dark and photographed immediately, because the yolks darken rapidly.


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
C. Moens
Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Probe Synthesis
CSH Protocols, August 1, 2008; 2008(9): pdb.prot5036 - pdb.prot5036.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
C. Moens
Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Hybridization
CSH Protocols, August 1, 2008; 2008(9): pdb.prot5037 - pdb.prot5037.
[Abstract] [Full Text]