Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.ip63

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Choosing the Sequence of dsRNA for RNAi in Mouse

Petr Svoboda

Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic

Corresponding author (svobodap{at}img.cas.cz)


INTRODUCTION

RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.


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