Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5117

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Generation of High-Titer Lentivirus for the Production of Transgenic Quail

Greg Poynter, David Huss, and Rusty Lansford1

Division of Biology and the Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA

1Corresponding author (rusty{at}caltech.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes how to generate high-titer lentivirus for the production of transgenic Japanese quail. The virus is pseudotyped with vesicular stomatitis virus with the envelope G glycoprotein (VSV-g), which gives a broad infectious range and allows concentration of viral supernatants by ultracentrifugation. Using this method, we typically produce titers >1 x 108 transforming units (TU)/mL and recommend using a virus with a titer at least this high for in vivo work.


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Related Article

Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians
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CSH Protocols 2009: 112. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
G. Poynter, D. Huss, and R. Lansford
Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians
CSH Protocols, January 1, 2009; 2009(1): pdb.emo112 - pdb.emo112.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
G. Poynter, D. Huss, and R. Lansford
Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5118 - pdb.prot5118.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
G. Poynter, D. Huss, and R. Lansford
Screening for Transgenic Japanese Quail Offspring
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5119 - pdb.prot5119.
[Abstract] [Full Text]