Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5118

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Injection of Lentivirus into Stage-X Blastoderm for the Production of Transgenic Quail

Greg Poynter, David Huss, and Rusty Lansford1

Division of Biology and the Biological Imaging Center, Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA

1Corresponding author (rusty{at}caltech.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes how to generate transgenic quail by injecting a lentiviral vector into freshly laid Japanese quail eggs at stage X. The lentivirus infects primordial germ cells originating in the area pellucida.


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Related Article

Japanese Quail: An Efficient Animal Model for the Production of Transgenic Avians
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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
G. Poynter, D. Huss, and R. Lansford
Generation of High-Titer Lentivirus for the Production of Transgenic Quail
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5117 - pdb.prot5117.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
G. Poynter, D. Huss, and R. Lansford
Screening for Transgenic Japanese Quail Offspring
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5119 - pdb.prot5119.
[Abstract] [Full Text]