Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5122

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Culturing Lamprey Embryos

Natalya Nikitina, Marianne Bronner-Fraser1, and Tatjana Sauka-Spengler1

Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA

1Corresponding authors (mbronner{at}caltech.edu and spengler{at}caltech.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Lampreys are one of the most basal animals in which many of the true vertebrate characteristics (e.g., neural crest, placodes, segmented brain, skull, paired sensory organs, pharyngeal skeleton) are present. Studying the molecular and developmental mechanisms responsible for the formation of these structures in lamprey and higher vertebrates can provide insight into how these vertebrate characteristics evolved. The relative ease of obtaining mature adults and embryos makes this animal an ideal model for investigations into early vertebrate evolution. In addition, studies of features that are unique to lampreys can provide insights into mechanisms of parallel evolution. This protocol describes how to produce lamprey embryos by collecting sperm and eggs from mature lampreys, performing fertilization, and culturing the embryos through to the desired developmental stage.


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
N. Nikitina, M. Bronner-Fraser, and T. Sauka-Spengler
The Sea Lamprey Petromyzon marinus: A Model for Evolutionary and Developmental Biology
CSH Protocols, January 1, 2009; 2009(1): pdb.emo113 - pdb.emo113.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. Nikitina, M. Bronner-Fraser, and T. Sauka-Spengler
Microinjection of RNA and Morpholino Oligos into Lamprey Embryos
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5123 - pdb.prot5123.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. Nikitina, M. Bronner-Fraser, and T. Sauka-Spengler
DiI Cell Labeling in Lamprey Embryos
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5124 - pdb.prot5124.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. Nikitina, M. Bronner-Fraser, and T. Sauka-Spengler
Whole-Mount In Situ Hybridization on Lamprey Embryos
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5125 - pdb.prot5125.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. Nikitina, M. Bronner-Fraser, and T. Sauka-Spengler
Immunostaining of Whole-Mount and Sectioned Lamprey Embryos
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5126 - pdb.prot5126.
[Abstract] [Full Text]