Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5127

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rehm, E. J.
Right arrow Articles by Patel, N. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rehm, E. J.
Right arrow Articles by Patel, N. H.
Related Collections
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrowRelated Article
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Fixation and Dissection of Parhyale hawaiensis Embryos

E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel1

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3140, USA
Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA
Howard Hughes Medical Institute, University of California, Berkeley, CA 94720-3140, USA

1Corresponding author (nipam{at}uclink.berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

The great diversity of arthropod body plans, together with our detailed understanding of fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification of developmental patterns and hence the diversity of extant life. Crustaceans, in particular, show a remarkable range of morphologies and provide a useful outgroup to the insects. The amphipod crustacean Parhyale hawaiensis is becoming established as a model organism for developmental studies within the arthropods. This protocol describes the dissection and fixation of P. hawaiensis embryos. Embryonic tissue fixed in the following manner is suitable for in situ hybridization experiments to study mRNA expression or for immunocytochemistry to study protein localization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Crustacean Parhyale hawaiensis: A New Model for Arthropod Development
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
Cold Spring Harb Protoc 2009: 114. [Abstract] [Full Text]

Related Protocols

Injection of Parhyale hawaiensis Blastomeres with Fluorescently Labeled Tracers
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
Cold Spring Harb Protoc 2009: 5128. [Abstract] [Full Text]

Antibody Staining of Parhyale hawaiensis Embryos
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
Cold Spring Harb Protoc 2009: 5129. [Abstract] [Full Text]

In Situ Hybridization of Labeled RNA Probes to Fixed Parhyale hawaiensis Embryos
E. Jay Rehm, Roberta L. Hannibal, R. Crystal Chaw, Mario A. Vargas-Vila, and Nipam H. Patel
Cold Spring Harb Protoc 2009: 5130. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
E. J. Rehm, R. L. Hannibal, R. C. Chaw, M. A. Vargas-Vila, and N. H. Patel
The Crustacean Parhyale hawaiensis: A New Model for Arthropod Development
Cold Spring Harb Protoc, January 1, 2009; 2009(1): pdb.emo114 - pdb.emo114.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
E. J. Rehm, R. L. Hannibal, R. C. Chaw, M. A. Vargas-Vila, and N. H. Patel
Injection of Parhyale hawaiensis Blastomeres with Fluorescently Labeled Tracers
Cold Spring Harb Protoc, January 1, 2009; 2009(1): pdb.prot5128 - pdb.prot5128.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
E. J. Rehm, R. L. Hannibal, R. C. Chaw, M. A. Vargas-Vila, and N. H. Patel
Antibody Staining of Parhyale hawaiensis Embryos
Cold Spring Harb Protoc, January 1, 2009; 2009(1): pdb.prot5129 - pdb.prot5129.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
E. J. Rehm, R. L. Hannibal, R. C. Chaw, M. A. Vargas-Vila, and N. H. Patel
In Situ Hybridization of Labeled RNA Probes to Fixed Parhyale hawaiensis Embryos
Cold Spring Harb Protoc, January 1, 2009; 2009(1): pdb.prot5130 - pdb.prot5130.
[Abstract] [Full Text]