Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5133

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Microinjection of dsRNA into Mouse One-Cell Embryos

Paula Stein

Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

Corresponding author (steinpau{at}sas.upenn.edu)


INTRODUCTION

RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing in a number of model systems. The following protocol describes delivery of a double-stranded RNA (dsRNA) of choice into mouse one-cell embryos by microinjection. Microinjected embryos can be cultured for up to 4 d, i.e., to the blastocyst stage. The efficiency of knockdown by RNAi can be assayed by quantitative reverse transcriptase (RT)-PCR (qPCR).


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This article has been cited by other articles:


Home page
 CSH ProtocolsHome page
P. Svoboda and P. Stein
RNAi Experiments in Mouse Oocytes and Early Embryos
CSH Protocols, January 1, 2009; 2009(1): pdb.top56 - pdb.top56.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
P. Svoboda
Preparation of dsRNA for Microinjection Experiments in Mouse
CSH Protocols, January 1, 2009; 2009(1): pdb.prot5131 - pdb.prot5131.
[Abstract] [Full Text]