Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.emo134

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Werren, J. H.
Right arrow Articles by Loehlin, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Werren, J. H.
Right arrow Articles by Loehlin, D. W.
Related Collections
Right arrow Bioinformatics/Genomics, general
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Evolution
Right arrow Evolutionary Development (Evo-Devo)
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

emoEmerging Model Organisms

The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics

John H. Werren1 and David W. Loehlin

Department of Biology, University of Rochester, Rochester, NY 14627, USA

1Corresponding author (werr{at}mail.rochester.edu).


INTRODUCTION

Nasonia is a complex of four closely related species of wasps that is rapidly emerging as a model for evolutionary and developmental genetics. It has several features that make it an excellent genetic system, including a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. The form of sex determination, called "haplodiploidy," makes Nasonia particularly suitable as a genetic tool. Females are diploid and develop from fertilized eggs, whereas males are haploid and develop from unfertilized eggs. This allows geneticists to exploit many of the advantages of haploid genetics in an otherwise complex eukaryotic organism. Nasonia readily inbreeds, permitting production of isogenic lines, and the four species in the genus are interfertile (after removal of the endosymbiont Wolbachia), facilitating movement of genes between the species for efficient positional cloning of quantitative trait loci (QTL). Genome sequencing of the genetic model Nasonia vitripennis and two interfertile species, Nasonia giraulti and Nasonia longicornis, is now completed. This genome project provides a wealth of interspecies polymorphisms (e.g., single nucleotide polymorphisms [SNPs], insertion-deletions [indels], microsatellites) to facilitate positional cloning of genes involved in species differences in behavior, morphology, and development. Advances in the genetics of this system also open a path for improvement of parasitoid insects as agents of pest control.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Strain Maintenance of Nasonia vitripennis (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5307.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Rearing Sarcophaga bullata Fly Hosts for Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5308.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Egg Collection for Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5309.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Virgin Collection and Haplodiploid Crossing Methods in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5310.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren, D. W. Loehlin, and J. D. Giebel
Larval RNAi in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5311.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Curing Wolbachia Infections in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5312.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Field Collection of Nasonia (Parasitoid Wasp) Using Baits
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5313.
[Abstract] [Full Text]