Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.ip74

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bustamante, C.
Right arrow Articles by Moffitt, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bustamante, C.
Right arrow Articles by Moffitt, J. R.
Related Collections
Right arrow Cell Biology, general
Right arrow Imaging/Microscopy, general
Right arrow Molecular Biology, general
Right arrow Cell Imaging
Right arrowRelated Articles
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

information_panelInformation Panel

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Data Collection and Instrument Calibration

Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt

Adapted from Single-Molecule Techniques (eds. Selvin and Ha). CSHL Press, Cold Spring Harbor, NY, USA, 2008.


INTRODUCTION

Optical traps or "optical tweezers" have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Articles

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Managing Environmental Noise
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc 2009: 72. [Abstract] [Full Text]

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Instrument Design
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc 2009: 73. [Abstract] [Full Text]

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Minimizing the Influence of Measurement Noise
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc 2009: 75. [Abstract] [Full Text]

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Alignment of Instrument Components
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc 2009: 76. [Abstract] [Full Text]

High-Resolution Dual-Trap Optical Tweezers with Differential Detection: An Introduction
Carlos Bustamante, Yann R. Chemla, and Jeffrey R. Moffitt
Cold Spring Harb Protoc 2009: 60. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
C. Bustamante, Y. R. Chemla, and J. R. Moffitt
High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Managing Environmental Noise
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.ip72.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Bustamante, Y. R. Chemla, and J. R. Moffitt
High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Instrument Design
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.ip73.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Bustamante, Y. R. Chemla, and J. R. Moffitt
High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Minimizing the Influence of Measurement Noise
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.ip75.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Bustamante, Y. R. Chemla, and J. R. Moffitt
High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Alignment of Instrument Components
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.ip76.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
C. Bustamante, Y. R. Chemla, and J. R. Moffitt
High-Resolution Dual-Trap Optical Tweezers with Differential Detection: An Introduction
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.top60.
[Abstract] [Full Text]