Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5298

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Fixation and Paraffin-Embedding of Mouse Tissues for GFP Visualization

Hugo Vankelecom

Adapted from Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. CSHL Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Green fluorescent protein (GFP) and its variants are used in many applications, including protein localization, high-throughput screening, and gene discovery. Transgenes that use GFP-based proteins as a reporter provide a convenient way to follow protein expression over the course of development and disease progression in the mouse. The basic principle of visualizing fluorescent proteins is the same as for the fluorophores used in fluorescent microscopy. This protocol describes a method for fixation and paraffin-embedding of fresh mouse embryos or tissue for use in GFP microscopy.


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