Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5301

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Fixing and Sectioning Tissue from the Plant Kalanchoë daigremontiana

Helena Garcês and Neelima Sinha1

Department of Plant Biology, University of California, Davis, CA 95616, USA

1Corresponding author (nrsinha{at}ucdavis.edu).


INTRODUCTION

Tissue samples from Kalanchoë daigremontiana can be fixed for scanning electron microscopy (SEM), histology, and in situ hybridization using several common steps described in this protocol. All steps should be performed in the same manner for all three methods unless otherwise noted.


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Related Article

The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc 2009: 133. [Abstract] [Full Text]



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H. Garces and N. Sinha
The 'Mother of Thousands' (Kalanchoe daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Cold Spring Harb Protoc, October 1, 2009; 2009(10): pdb.emo133 - pdb.emo133.
[Abstract] [Full Text]