Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5302

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In Situ Hybridization in the Plant Kalanchoë daigremontiana

Helena Garcês and Neelima Sinha1

Department of Plant Biology, University of California, Davis, CA 95616, USA

1Corresponding author (nrsinha{at}ucdavis.edu).


INTRODUCTION

Here we describe in detail the detection of gene expression in plant tissues of Kalanchoë daigremontiana by in situ hybridization analyses. Included are methods for making RNA transcript probes, probe-tissue hybridization, and detection of antisense RNA probes. The in situ hybridization technique is used to determine which cells or group of cells in particular tissue(s) express a gene of interest.


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Related Article

The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
CSH Protocols 2009: 133. [Abstract] [Full Text]



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H. Garces and N. Sinha
The 'Mother of Thousands' (Kalanchoe daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
CSH Protocols, October 1, 2009; 2009(10): pdb.emo133 - pdb.emo133.
[Abstract] [Full Text]