Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5303

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Transformation of the Plant Kalanchoë daigremontiana using Agrobacterium tumefaciens

Helena Garcês and Neelima Sinha1

Department of Plant Biology, University of California, Davis, CA 95616, USA

1Corresponding author (nrsinha{at}ucdavis.edu).


INTRODUCTION

Kalanchoë daigremontiana can be stably transformed using the Agrobacterium tumefaciens-mediated T-DNA transfer method, as described here. Sterilized plant tissue is cocultivated with an A. tumefaciens suspension, transformants are selected and the shoots are grown in rooting medium and then in soil. Plant phenotypes can be examined ~3 mo after transfer of plants to soil.


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Related Article

The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
CSH Protocols 2009: 133. [Abstract] [Full Text]



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H. Garces and N. Sinha
The 'Mother of Thousands' (Kalanchoe daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
CSH Protocols, October 1, 2009; 2009(10): pdb.emo133 - pdb.emo133.
[Abstract] [Full Text]