Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5304

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protocolProtocol

Extraction of DNA from the Plant Kalanchoë daigremontiana

Helena Garcês and Neelima Sinha1

Department of Plant Biology, University of California, Davis, CA 95616, USA

1Corresponding author (nrsinha{at}ucdavis.edu).


INTRODUCTION

This protocol describes how to isolate genomic DNA from leaves and stems of Kalanchoë daigremontiana. The procedure can be applied to adult leaves, but it is best to use younger leaves because they have fewer secondary metabolites and polysaccharides, which can interfere with the DNA extraction. The resulting DNA can be used for polymerase chain reactions (PCRs), Southern blots, or other applications.


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Related Article

The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc 2009: 133. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
H. Garces and N. Sinha
The 'Mother of Thousands' (Kalanchoe daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.emo133.
[Abstract] [Full Text]