Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5305

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protocolProtocol

Extraction of RNA from the Plant Kalanchoë daigremontiana

Helena Garcês and Neelima Sinha1

Department of Plant Biology, University of California, Davis, CA 95616, USA.

1Corresponding author (nrsinha{at}ucdavis.edu).


INTRODUCTION

This protocol describes how to isolate total RNA from several tissues of Kalanchoë daigremontiana. Total RNA can be isolated by using the TRI-reagent method, scaled up for processing 2 g of tissue, or by using the protocol described here, which gives higher concentrations of high quality RNA. The resulting RNA can be used for various applications including generation of cDNA for reverse transcriptase-PCR (RT-PCR), Northern blots, or other purposes.


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Related Article

The ‘Mother of Thousands’ (Kalanchoë daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Helena Garcês and Neelima Sinha
Cold Spring Harb Protoc 2009: 133. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
H. Garces and N. Sinha
The 'Mother of Thousands' (Kalanchoe daigremontiana): A Plant Model for Asexual Reproduction and CAM Studies
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.emo133.
[Abstract] [Full Text]