Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5308

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Werren, J. H.
Right arrow Articles by Loehlin, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Werren, J. H.
Right arrow Articles by Loehlin, D. W.
Related Collections
Right arrow Genetics, general
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Evolution
Right arrow Evolutionary Development (Evo-Devo)
Right arrowRelated Article
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Rearing Sarcophaga bullata Fly Hosts for Nasonia (Parasitoid Wasp)

John H. Werren1 and David W. Loehlin

Department of Biology, University of Rochester, Rochester, NY 14627, USA

1Corresponding author (werr{at}mail.rochester.edu).


INTRODUCTION

Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. Nasonia vitripennis is a parasitoid of a number of calliphorid flies, such as Sarcophaga bullata, allowing investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. S. bullata are relatively large, increasing the offspring yield that a single N. vitripennis female can produce. They are also easily reared in the lab if proper ventilation is available. This protocol describes the methods to collect and raise S. bullata larvae that will be used as hosts for N. vitripennis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc 2009: 134. [Abstract] [Full Text]

Related Protocol

Field Collection of Nasonia (Parasitoid Wasp) Using Baits
John H. Werren and David W. Loehlin
Cold Spring Harb Protoc 2009: 5313. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.emo134.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Strain Maintenance of Nasonia vitripennis (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5307.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Egg Collection for Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5309.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Virgin Collection and Haplodiploid Crossing Methods in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5310.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren, D. W. Loehlin, and J. D. Giebel
Larval RNAi in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5311.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Curing Wolbachia Infections in Nasonia (Parasitoid Wasp)
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5312.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
Field Collection of Nasonia (Parasitoid Wasp) Using Baits
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.prot5313.
[Abstract] [Full Text]