Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5310

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Virgin Collection and Haplodiploid Crossing Methods in Nasonia (Parasitoid Wasp)

John H. Werren1 and David W. Loehlin

Department of Biology, University of Rochester, Rochester, NY 14627, USA

1Corresponding author (werr{at}mail.rochester.edu).


INTRODUCTION

This protocol covers most procedures used by the Nasonia (wasp) geneticist, including virgin collection. Nasonia are easily sexed as pupae, so collecting virgins can easily be performed within a window of several days. The female-specific ovipositor is visible at even the earliest pupal stages as a longitudinal ridge on the ventral posterior abdomen. To prolong generations, yellow pupae can be stored at 4°C-8°C for up to 2 mo and adults for 2-3 wk. In the example presented here, haplodiploid crossing methods are used to make a new Nasonia vitripennis mutant homozygous, using an aunt/nephew mating approach. The protocol can be extended to inbreeding strains. Inbreeding depression generally is not a problem in Nasonia unless the mutation itself is female-sterile. Mother/son mating and sib mating are also reasonable approaches to inbreeding.


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The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
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Cold Spring Harb Protoc 2009: 134. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
Cold Spring Harb Protoc, October 1, 2009; 2009(10): pdb.emo134 - pdb.emo134.
[Abstract] [Full Text]