Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5311

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protocolProtocol

Larval RNAi in Nasonia (Parasitoid Wasp)

John H. Werren2, David W. Loehlin, and Jonathan D. Giebel1

Department of Biology, University of Rochester, Rochester, NY 14627, USA

1Present address: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

2Corresponding author (werr{at}mail.rochester.edu).


INTRODUCTION

Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes a method to use RNA interference (RNAi) to knock down genes in Nasonia larvae. Unlike in Drosophila, RNAi in Nasonia is systemic. In the example presented here, adult red-eye-color phenotypes are produced by injecting double-stranded RNA (dsRNA) against the eye color gene cinnabar into last-instar Nasonia larvae.


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 Cold Spring Harb ProtocHome page
J. H. Werren and D. W. Loehlin
The Parasitoid Wasp Nasonia: An Emerging Model System with Haploid Male Genetics
Cold Spring Harb Protoc, October 1, 2009; 2009(10): 10.1101/pdb.emo134.
[Abstract] [Full Text]