Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5320

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Confocal Immunocytochemistry of Embryonic and Juvenile Hawaiian Bobtail Squid (Euprymna scolopes) Tissues

Patricia N. Lee1,2, Margaret J. McFall-Ngai3, Patrick Callaerts4, and H. Gert de Couet1,5

1 Department of Zoology, University of Hawaii at Manoa, Honolulu, HI 96822, USA
2 Kewalo Marine Laboratory, Pacific Biosciences Research Center/University of Hawaii at Manoa, Honolulu, HI 96813, USA
3 Department of Medical Microbiology, University of Wisconsin-Madison, Madison, WI 53706-1521, USA
4 Laboratory of Developmental Genetics, K.U. Leuven and VIB, B-3000 Leuven, Belgium

5Corresponding author (couet{at}hawaii.edu).


INTRODUCTION

The Hawaiian bobtail squid Euprymna scolopes is a cephalopod whose small size, short lifespan, rapid growth, and year-round availability make it suitable as a model organism. This protocol describes the preparation of whole juvenile squids by whole-mount immunocytochemistry for visualization by confocal microscopy.


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P. N. Lee, M. J. McFall-Ngai, P. Callaerts, and H. G. de Couet
The Hawaiian Bobtail Squid (Euprymna scolopes): A Model to Study the Molecular Basis of Eukaryote-Prokaryote Mutualism and the Development and Evolution of Morphological Novelties in Cephalopods
CSH Protocols, November 1, 2009; 2009(11): pdb.emo135 - pdb.emo135.
[Abstract] [Full Text]