Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5323

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protocolProtocol

Culture of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos and Observation of Normal Development

Patricia N. Lee1,2, Patrick Callaerts3, and H. Gert de Couet1,4

1 Department of Zoology, University of Hawaii at Manoa, Honolulu, HI 96822, USA
2 Kewalo Marine Laboratory, Pacific Biosciences Research Center/University of Hawaii at Manoa, Honolulu, HI 96813, USA
3 Laboratory of Developmental Genetics, K.U. Leuven and VIB, B-3000 Leuven, Belgium

4Corresponding author (couet{at}hawaii.edu).


INTRODUCTION

The ability to rear Hawaiian bobtail squid (Euprymna scolopes) embryos under controlled environmental conditions is a basic and necessary tool for developmental studies. It negates the need to know when fertilization occurred, allows correlation of the phase of development with the time of development (thereby facilitating collection of embryos at specific stages), and allows comparisons between cephalopod species. Embryonic development in E. scolopes is robust over a range of temperatures, is relatively rapid (~21 d), and proceeds normally under laboratory conditions at ambient temperature (27°C-29°C). Here we present methods for maintaining E. scolopes embryos in culture from cleavage through hatching, as well as observing and recording live or freshly fixed embryos under a dissecting microscope.


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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
P. N. Lee, P. Callaerts, and H. G. de Couet
The Embryonic Development of the Hawaiian Bobtail Squid (Euprymna scolopes)
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.ip77 - pdb.ip77.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
P. N. Lee, M. J. McFall-Ngai, P. Callaerts, and H. G. de Couet
The Hawaiian Bobtail Squid (Euprymna scolopes): A Model to Study the Molecular Basis of Eukaryote-Prokaryote Mutualism and the Development and Evolution of Morphological Novelties in Cephalopods
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.emo135 - pdb.emo135.
[Abstract] [Full Text]