Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5326

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Post-Flask Management of Yam (Dioscorea) Plantlets

Hodeba D. Mignouna1, Mathew M. Abang2, Robert Asiedu3, and R. Geeta4,5

1 African Agricultural Technology Foundation, Nairobi 00100, Kenya
2 AVRDC-The World Vegetable Center, Regional Center for Africa, Duluti, Arusha, Tanzania
3 International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria
4 Department of Ecology and Evolution, State University of New York, Stony Brook, NY 11794, USA

5Corresponding author (geeta{at}life.bio.sunysb.edu).


INTRODUCTION

This protocol describes how to harden or acclimatize tissue culture-derived yam (Dioscorea) plantlets before transplanting them in the soil. Briefly, the plantlets are first put in pots with a vermiculite and peat moss mixture, and the pots are placed in a humidity chamber, where they are sprayed and watered daily. After 2 wk, the plants are transplanted to pots or plastic bags with ordinary or sterile soil and watered daily. After another 2 wk, they are transplanted into the field.


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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
H. D. Mignouna, M. M. Abang, R. Asiedu, and R. Geeta
True Yams (Dioscorea): A Biological and Evolutionary Link between Eudicots and Grasses
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.emo136 - pdb.emo136.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
H. D. Mignouna, M. M. Abang, R. Asiedu, and R. Geeta
Culturing Meristematic Tissue and Node Cuttings from Yams (Dioscorea)
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.prot5325 - pdb.prot5325.
[Abstract] [Full Text]