Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5327

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protocolProtocol

Producing Yam (Dioscorea) Seeds through Controlled Crosses

Hodeba D. Mignouna1, Mathew M. Abang2, Robert Asiedu3, and R. Geeta4,5

1 African Agricultural Technology Foundation, Nairobi 00100, Kenya
2 AVRDC-The World Vegetable Center, Regional Center for Africa, Duluti, Arusha, Tanzania
3 International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria
4 Department of Ecology and Evolution, State University of New York, Stony Brook, NY 11794, USA

5Corresponding author (geeta{at}life.bio.sunysb.edu).


INTRODUCTION

This protocol describes how to produce yam (Dioscorea) seeds through controlled pollination of flowers on female plants. Pollination by flower thrips takes place naturally in normal yam-growing environments in the presence of flowering male and female genotypes, although it is not efficient. This protocol leads to the production of hybrid seeds of known parentage for genetic studies and use in breeding programs.


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Home page
 Cold Spring Harb ProtocHome page
H. D. Mignouna, M. M. Abang, R. Asiedu, and R. Geeta
True Yams (Dioscorea): A Biological and Evolutionary Link between Eudicots and Grasses
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.emo136 - pdb.emo136.
[Abstract] [Full Text]