Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5328

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protocolProtocol

Extraction of DNA from Yam (Dioscorea) Leaves

Hodeba D. Mignouna1, Mathew M. Abang2, Robert Asiedu3, and R. Geeta4,5

1 African Agricultural Technology Foundation, Nairobi 00100, Kenya
2 AVRDC-The World Vegetable Center, Regional Center for Africa, Duluti, Arusha, Tanzania
3 International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria
4 Department of Ecology and Evolution, State University of New York, Stony Brook, NY 11794, USA

5Corresponding author (geeta{at}life.bio.sunysb.edu).


INTRODUCTION

This protocol describes how to extract DNA from the leaves of true yams (Dioscorea) for polymerase chain reaction (PCR) and other analyses.


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Home page
 Cold Spring Harb ProtocHome page
H. D. Mignouna, M. M. Abang, R. Asiedu, and R. Geeta
True Yams (Dioscorea): A Biological and Evolutionary Link between Eudicots and Grasses
Cold Spring Harb Protoc, November 1, 2009; 2009(11): pdb.emo136 - pdb.emo136.
[Abstract] [Full Text]