Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5339

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Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Cleavage Stages and Blastocysts

Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree1

Department of Zoology, The University of Melbourne, Victoria 3010, Australia

1Corresponding author (m.renfree{at}unimelb.edu.au).


INTRODUCTION

This protocol describes a method for whole-mount immunofluorescence staining of early stages of tammar wallaby (Macropus eugenii) development, including cleavage and unilaminar blastocysts. Conceptuses of these stages are surrounded by a shell coat, which must be removed before staining to ensure proper penetration of all solutions.


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Related Article

The Tammar Wallaby, Macropus eugenii: A Model Kangaroo for the Study of Developmental and Reproductive Biology
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc 2009: 137. [Abstract] [Full Text]

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Collection, Handling, Fixation, and Processing of Tammar Wallaby (Macropus eugenii) Embryos
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Immunohistochemical Staining of Sectioned Tammar Wallaby (Macropus eugenii) Tissue
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Home page
 Cold Spring Harb ProtocHome page
D. Hickford, S. Frankenberg, and M. B. Renfree
The Tammar Wallaby, Macropus eugenii: A Model Kangaroo for the Study of Developmental and Reproductive Biology
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo137.
[Abstract] [Full Text]