Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5340

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Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Peri-gastrulation Embryos

Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree1

Department of Zoology, The University of Melbourne, Victoria 3010, Australia

1Corresponding author (m.renfree{at}unimelb.edu.au).


INTRODUCTION

This protocol describes a method for whole-mount immunohistochemical staining of tammar wallaby (Macropus eugenii) peri-gastrulation stage embryos using the avidin-biotin complex system. Tammar embryos are ideal for whole-mount immunostaining because of their morphology: They develop in a planar manner on the surface of the embryonic vesicle and are not covered by any extraembryonic membranes until amnion formation (which occurs during mid-somitogenesis). Tammar embryos are surrounded by a transparent shell coat, which must be opened prior to staining to ensure penetration of solutions and reagents.


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Related Article

The Tammar Wallaby, Macropus eugenii: A Model Kangaroo for the Study of Developmental and Reproductive Biology
Danielle Hickford, Stephen Frankenberg, and Marilyn B. Renfree
Cold Spring Harb Protoc 2009: 137. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
D. Hickford, S. Frankenberg, and M. B. Renfree
The Tammar Wallaby, Macropus eugenii: A Model Kangaroo for the Study of Developmental and Reproductive Biology
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo137.
[Abstract] [Full Text]