Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5344

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Isolation of Sea Squirt (Ciona) Gametes, Fertilization, Dechorionation, and Development

Lionel Christiaen1,2, Eileen Wagner, Weiyang Shi, and Michael Levine2

Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA

1 Present address: Center for Developmental Genetics, Department of Biology, Faculty of Arts and Science, New York University, New York, NY 10003, USA.

2Corresponding authors (mlevine{at}berkeley.edu); (lc121{at}nyu.edu).


INTRODUCTION

This protocol is the starting point for most manipulations that are used to study the sea squirt (Ciona) embryo, including in situ hybridization, the microinjection of morpholino oligos, and the electroporation of transgenic DNAs. Ciona eggs and embryos are exquisitely sensitive to even trace amounts of detergent; therefore, it is strongly advised to designate a soap-free workspace for embryo culture. Any solutions that come into contact with embryos should be prepared in absolutely clean glassware with the highest quality water.


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Related Article

The Sea Squirt Ciona intestinalis
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc 2009: 138. [Abstract] [Full Text]

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Electroporation of Transgenic DNAs in the Sea Squirt Ciona
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Microinjection of Morpholino Oligos and RNAs in Sea Squirt (Ciona) Embryos
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
The Sea Squirt Ciona intestinalis
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo138.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Electroporation of Transgenic DNAs in the Sea Squirt Ciona
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5345.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Microinjection of Morpholino Oligos and RNAs in Sea Squirt (Ciona) Embryos
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5347.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Whole-Mount In Situ Hybridization on Sea Squirt (Ciona intestinalis) Embryos
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5348.
[Abstract] [Full Text]