Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5345

This Protocol
Right arrow Full Text
Right arrow Discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christiaen, L.
Right arrow Articles by Levine, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christiaen, L.
Right arrow Articles by Levine, M.
Related Collections
Right arrow Cell Biology, general
Right arrow Labeling for Imaging
Right arrow Laboratory Organisms, general
Right arrow Molecular Biology, general
Right arrow Analysis of Gene Expression
Right arrow Analysis of Gene Expression, general
Right arrow Transgenic Technology, general
Right arrow Transgenics, Other Organisms
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrow Evolution
Right arrow Evolutionary Development (Evo-Devo)
Right arrow DNA Delivery/Gene Transfer
Right arrow DNA Delivery/Gene Transfer, general
Right arrow Non-Viral Methods
Right arrow Use of Reporter Genes
Right arrowRelated Article
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Electroporation of Transgenic DNAs in the Sea Squirt Ciona

Lionel Christiaen1,2, Eileen Wagner, Weiyang Shi, and Michael Levine2

Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA

1 Present address: Center for Developmental Genetics, Department of Biology, Faculty of Arts and Science, New York University, New York, NY 10003, USA.

2Corresponding authors (mlevine{at}berkeley.edu); (lc121{at}nyu.edu).


INTRODUCTION

The electroporation method described here is probably the mainstay of sea squirt (Ciona) research, because the utility of transgene expression in staged embryo populations enables a wide array of biological questions to be addressed. It allows rapid identification and characterization of cis-regulatory DNA, such as tissue-specific enhancers. Electroporation of plasmids expressing fluorescent reporter genes permits live imaging and lineage tracing. Finally, structure-function relationships can be examined by expressing dominant-negative or constitutively active forms of specific proteins using appropriate cell-specific enhancers.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

The Sea Squirt Ciona intestinalis
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc 2009: 138. [Abstract] [Full Text]

Related Protocol

Isolation of Sea Squirt (Ciona) Gametes, Fertilization, Dechorionation, and Development
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc 2009: 5344. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
The Sea Squirt Ciona intestinalis
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo138.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Isolation of Sea Squirt (Ciona) Gametes, Fertilization, Dechorionation, and Development
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5344.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
X-gal Staining of Electroporated Sea Squirt (Ciona) Embryos
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5346.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Isolation of Individual Cells and Tissues from Electroporated Sea Squirt (Ciona) Embryos by Fluorescence-Activated Cell Sorting (FACS)
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5349.
[Abstract] [Full Text]