Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5346

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X-gal Staining of Electroporated Sea Squirt (Ciona) Embryos

Lionel Christiaen1,2, Eileen Wagner, Weiyang Shi, and Michael Levine2

Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA

1 Present address: Center for Developmental Genetics, Department of Biology, Faculty of Arts and Science, New York University, New York, NY 10003, USA.

2Corresponding authors (mlevine{at}berkeley.edu); (lc121{at}nyu.edu).


INTRODUCTION

This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.


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The Sea Squirt Ciona intestinalis
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
The Sea Squirt Ciona intestinalis
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo138.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
Microinjection of Morpholino Oligos and RNAs in Sea Squirt (Ciona) Embryos
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.prot5347.
[Abstract] [Full Text]