Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5348

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Whole-Mount In Situ Hybridization on Sea Squirt (Ciona intestinalis) Embryos

Lionel Christiaen1,2, Eileen Wagner, Weiyang Shi, and Michael Levine2

Molecular and Cell Biology Department, University of California, Berkeley, California 94720, USA

1 Present address: Center for Developmental Genetics, Department of Biology, Faculty of Arts and Science, New York University, New York, NY 10003, USA.

2Corresponding authors (mlevine{at}berkeley.edu); (lc121{at}nyu.edu).


INTRODUCTION

This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.


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Related Article

The Sea Squirt Ciona intestinalis
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc 2009: 138. [Abstract] [Full Text]

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Isolation of Sea Squirt (Ciona) Gametes, Fertilization, Dechorionation, and Development
Lionel Christiaen, Eileen Wagner, Weiyang Shi, and Michael Levine
Cold Spring Harb Protoc 2009: 5344. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
L. Christiaen, E. Wagner, W. Shi, and M. Levine
The Sea Squirt Ciona intestinalis
Cold Spring Harb Protoc, December 1, 2009; 2009(12): 10.1101/pdb.emo138.
[Abstract] [Full Text]