Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.emo116

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emoEmerging Model Organisms

The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa

Nicole King1,2,3, Susan L. Young1, Monika Abedin1, Martin Carr4, and Barry S.C. Leadbeater5,6

1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
2Department of Integrative Biology, University of California, Berkeley, Berkeley, CA 94704, USA
3Canadian Institute for Advanced Research, Toronto, Ontario M5G 1Z8, Canada
4Department of Biology, University of York, York YO10 5YW, United Kingdom
5School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom

6Corresponding author (b.s.c.leadbeater{at}bham.ac.uk)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Choanoflagellates are the closest living relatives of the metazoa, and the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins. These heterotrophic flagellates are a cosmopolitan group of small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Monosiga brevicollis, a marine species, has emerged as a representative of the group because it is easily grown and manipulated in the laboratory and was the subject of a recently completed genome project. Because of the similarity in morphology and ecology shown across the order Choanoflagellida, the protocols mentioned in this article should be transferable to most choanoflagellate species.


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 CSH ProtocolsHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
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N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
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CSH Protocols, February 1, 2009; 2009(2): pdb.prot5151 - pdb.prot5151.
[Abstract] [Full Text]

Home page
 CSH ProtocolsHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
CSH Protocols, February 1, 2009; 2009(2): pdb.prot5152 - pdb.prot5152.
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Home page
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N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
CSH Protocols, February 1, 2009; 2009(2): pdb.prot5153 - pdb.prot5153.
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 CSH ProtocolsHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
Separation of Choanoflagellate and Bacterial Genomic DNA
CSH Protocols, February 1, 2009; 2009(2): pdb.prot5154 - pdb.prot5154.
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