Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5140

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Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens

David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano1

Department of Biology, Washington University, St. Louis, MO 63130, USA

1Corresponding author (rsq{at}wustl.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes how to isolate individual protoplasts from young gametophyte tissue of the moss Physcomitrella patens and how to regenerate them into plants if desired. Protonemal tissue is grown on cellophane-overlay plates, harvested, and treated with 0.5% Driselase to produce protoplasts. After filtration and washing, the protoplasts are regenerated on solid medium.


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The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
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 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
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