Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5141

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Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion

David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano1

Department of Biology, Washington University, St. Louis, MO 63130, USA

1Corresponding author (rsq{at}wustl.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

As an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transgenic strains that are hygromycin- or G418-resistant by selecting hybrids on medium containing both antibiotics.


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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.emo115 - pdb.emo115.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.prot5140 - pdb.prot5140.
[Abstract] [Full Text]