Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5143

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Transformation of the Moss Physcomitrella patens Using Direct DNA Uptake by Protoplasts

David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano1

Department of Biology, Washington University, St. Louis, MO 63130, USA

1 Corresponding author (rsq{at}wustl.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes how to transform moss (Physcomitrella patens) protoplasts using polyethylene glycol (PEG)-mediated DNA uptake. The transformation rates for direct uptake by protoplasts of DNA with and without genomic sequence (a targeting construct) are typically 10–5 and 10–3, respectively. (These are the frequencies of stable transformants among regenerants surviving the transformation procedure.)


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The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
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Cold Spring Harb Protoc 2009: 115. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.emo115 - pdb.emo115.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.prot5140 - pdb.prot5140.
[Abstract] [Full Text]