Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5145

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protocolProtocol

Transformation of Moss Physcomitrella patens Gametophytes Using a Biolistic Projectile Delivery System

David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano1

Department of Biology, Washington University, St. Louis, MO 63130, USA

1Corresponding author (rsq{at}wustl.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This method makes use of biolistic bombardment to propel gold particles coated with DNA into protonemal tissue of the moss Physcomitrella patens. It is especially suitable for transient gene expression studies, but it can be used to obtain stable transformants.


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The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
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Cold Spring Harb Protoc 2009: 115. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.emo115 - pdb.emo115.
[Abstract] [Full Text]