Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5146

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Isolation of DNA, RNA, and Protein from the Moss Physcomitrella patens Gametophytes

David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano1

Department of Biology, Washington University, St. Louis, MO 63130, USA

1Corresponding author (rsq{at}wustl.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes a series of procedures for isolating nucleic acids (DNA and RNA) and proteins from moss (Physcomitrella patens) tissue. This series includes a rapid, small-scale procedure for isolating DNA, which results in genomic DNA that is only suitable for polymerase chain reaction (PCR), as well as a method for obtaining much larger amounts of genomic DNA suitable for Southern analysis. The latter method makes use of the Nucleon PhytoPure Genomic DNA Extraction Kit (GE Healthcare). The RNA extraction procedure uses the Plant RNA Isolation Mini Kit (Agilent) with some modifications. A method for extracting proteins from moss gametophytes is also presented.


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Related Article

The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc 2009: 115. [Abstract] [Full Text]

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Culturing the Moss Physcomitrella patens
David J. Cove, Pierre-François Perroud, Audra J. Charron, Stuart F. McDaniel, Abha Khandelwal, and Ralph S. Quatrano
Cold Spring Harb Protoc 2009: 5136. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
D. J. Cove, P.-F. Perroud, A. J. Charron, S. F. McDaniel, A. Khandelwal, and R. S. Quatrano
The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.emo115 - pdb.emo115.
[Abstract] [Full Text]