Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5152

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Rapid Preparation of Genomic DNA from Monosiga brevicollis and Other Choanoflagellates

Nicole King1,2,3, Susan L. Young1, Monika Abedin1, Martin Carr4, and Barry S.C. Leadbeater5,6

1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94704, USA
2Department of Integrative Biology, University of California, Berkeley, Berkeley, CA 94704, USA
3Canadian Institute for Advanced Research, Toronto, Ontario M5G 1Z8, Canada
4Department of Biology, University of York, York YO10 5YW, United Kingdom
5School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom

6Corresponding author (b.s.c.leadbeater{at}bham.ac.uk)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Choanoflagellates are heterotrophic nanoflagellates: small, colorless protozoa that are present in marine and freshwater environments as well as in hydrated soils. Because they are the closest living relatives of the metazoa, the study of their cell biology and genomes promises to provide new insights into metazoan ancestry and origins. This protocol describes a straightforward but crude isolation strategy for genomic DNA that has been effective on all tested choanoflagellate species. The resulting genomic DNA provides a suitable template for a variety of polymerase chain reaction (PCR) techniques, but is too impure for procedures that require very high-quality DNA such as Southern blotting.


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Related Article

The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa
Nicole King, Susan L. Young, Monika Abedin, Martin Carr, and Barry S.C. Leadbeater
Cold Spring Harb Protoc 2009: 116. [Abstract] [Full Text]

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Isolation of Single Choanoflagellate Cells from Field Samples and Establishment of Clonal Cultures
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Starting and Maintaining Monosiga brevicollis Cultures
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Cold Spring Harb Protoc 2009: 5148. [Abstract] [Full Text]

Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates
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Cold Spring Harb Protoc 2009: 5151. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
The Choanoflagellates: Heterotrophic Nanoflagellates and Sister Group of the Metazoa
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.emo116 - pdb.emo116.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
Preparation of Total RNA from Monosiga brevicollis and Other Choanoflagellates
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.prot5151 - pdb.prot5151.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
N. King, S. L. Young, M. Abedin, M. Carr, and B. S.C. Leadbeater
Preparation of High-Molecular-Weight Genomic DNA from Monosiga brevicollis and Other Choanoflagellates
Cold Spring Harb Protoc, February 1, 2009; 2009(2): pdb.prot5153 - pdb.prot5153.
[Abstract] [Full Text]