Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5163

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Fixation and Storage of Embryos from Short-Tailed Fruit Bat (Carollia perspicillata)

John J. Rasweiler, IV1, Chris J. Cretekos2, and Richard R. Behringer3,4

1 Department of Obstetrics and Gynecology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
2 Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA
3 Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA

4Corresponding author (rrb{at}mdanderson.org)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol describes fixation of embryos and tissues of the short-tailed fruit bat C. perspicillata. The fixed embryos can be stored at –20°C for prolonged periods and can be used for whole-mount in situ hybridization, whole-mount immunohistochemistry, or for embedding and sectioning in paraffin or frozen blocks for a variety of histological procedures.


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Related Article

The Short-Tailed Fruit Bat Carollia perspicillata: A Model for Studies in Reproduction and Development
John J. Rasweiler, IV, Chris J. Cretekos, and Richard R. Behringer
Cold Spring Harb Protoc 2009: 118. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
J. J. Rasweiler IV, C. J. Cretekos, and R. R. Behringer
The Short-Tailed Fruit Bat Carollia perspicillata: A Model for Studies in Reproduction and Development
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.emo118 - pdb.emo118.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
J. J. Rasweiler IV, C. J. Cretekos, and R. R. Behringer
Whole-Mount In Situ Hybridization of Short-Tailed Fruit Bat (Carollia perspicillata) Embryos with RNA Probes
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5164 - pdb.prot5164.
[Abstract] [Full Text]