Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5165

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Alcian Blue Staining of Cartilage of Short-Tailed Fruit Bat (Carollia perspicillata)

John J. Rasweiler, IV1, Chris J. Cretekos2, and Richard R. Behringer3,4

1 Department of Obstetrics and Gynecology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
2 Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA
3 Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA

4Corresponding author (rrb{at}mdanderson.org)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

This protocol is used to visualize the cartilaginous elements of the developing skeleton using alcian blue. It has been used with excellent results on the fruit bat Carollia perspicillata, as well as several other bat species at stages from CS 14 through CS 22. Staining has been shown to last at least 2 yr in benzyl alcohol:benzyl benzoate (BABB). However, staining has been observed to weaken during the course of months. It is therefore advisable to document results immediately.


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The Short-Tailed Fruit Bat Carollia perspicillata: A Model for Studies in Reproduction and Development
John J. Rasweiler, IV, Chris J. Cretekos, and Richard R. Behringer
Cold Spring Harb Protoc 2009: 118. [Abstract] [Full Text]

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Home page
 Cold Spring Harb ProtocHome page
J. J. Rasweiler IV, C. J. Cretekos, and R. R. Behringer
The Short-Tailed Fruit Bat Carollia perspicillata: A Model for Studies in Reproduction and Development
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.emo118 - pdb.emo118.
[Abstract] [Full Text]