Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5169

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Related Collections
Right arrow CSHL Molecular Embryology of the Mouse Course
Right arrow Cell Biology, general
Right arrow Visualization
Right arrow Visualization, general
Right arrow Laboratory Organisms, general
Right arrow Mouse
Right arrow Phenotypic Analysis
Right arrow Developmental Biology
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Legend icon

protocolProtocol

Alcian Blue Staining of the Mouse Fetal Cartilaginous Skeleton

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

Adapted from Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. CSHL Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

The vertebrate skeleton forms by endochondral and intramembranous bone formation. During endochondral bone formation, mesenchyme condensations give rise to cartilages that are eventually replaced by bone. However, there are some permanent cartilages that do not ossify, such as the cartilage of the trachea and articular cartilage of the joints, and intramembranous bone formation occurs directly without a cartilage template. This protocol is ideal for revealing the cartilaginous skeleton of mouse embryos between 12.5 and 16.5 days post-coitum (dpc). The cartilaginous skeleton is stained dark blue by alcian blue, whereas the other tissues of the embryo are made transparent by a clearing solution, benzyl alcohol/benzyl benzoate (BABB).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
J. J. Rasweiler IV, C. J. Cretekos, and R. R. Behringer
Alcian Blue Staining of Cartilage of Short-Tailed Fruit Bat (Carollia perspicillata)
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5165 - pdb.prot5165.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
D. Ovchinnikov
Alcian Blue/Alizarin Red Staining of Cartilage and Bone in Mouse
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5170 - pdb.prot5170.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. Nagy, M. Gertsenstein, K. Vintersten, and R. Behringer
Alizarin Red Staining of Post-natal Bone in Mouse
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5171 - pdb.prot5171.
[Abstract] [Full Text]