Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5175

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In Situ Hybridization Analysis of Embryonic Beak Tissue from Darwin’s Finches

Arhat Abzhanov

Department of Organismic and Evolutionary Biology/FAS Biological Laboratories 4105, Harvard University, Cambridge, MA 02138, USA

Corresponding author (aabzhano{at}genetics.med.harvard.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

The beaks of Darwin’s finches develop their distinct shapes during embryogenesis. To visualize where and when mRNA transcripts of genes involved in beak development are present during embryogenesis, this protocol describes how to analyze embryonic tissue samples using in situ hybridization. The principle of this technique is to use probes specific to mRNAs coding for proteins of interest to reveal how the corresponding genes are expressed in the beaks of different species of Darwin’s finches.


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Darwin’s Finches: Analysis of Beak Morphological Changes During Evolution
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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. Abzhanov
Darwin's Finches: Analysis of Beak Morphological Changes During Evolution
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.emo119 - pdb.emo119.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. Abzhanov
Collection of Embryos from Darwin's Finches (Thraupidae, Passeriformes)
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5174 - pdb.prot5174.
[Abstract] [Full Text]

Home page
 Cold Spring Harb ProtocHome page
A. Abzhanov
Microarray Analysis of Embryonic Beak mRNA from Darwin's Finches
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5176 - pdb.prot5176.
[Abstract] [Full Text]