Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5176

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Microarray Analysis of Embryonic Beak mRNA from Darwin’s Finches

Arhat Abzhanov

Department of Organismic and Evolutionary Biology/FAS Biological Laboratories 4105, Harvard University, Cambridge, MA 02138, USA

Corresponding author (aabzhano{at}genetics.med.harvard.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

In this protocol, microarray technology is used as a very sensitive and rapid method to identify genes that are potentially involved in beak development and morphology in Darwin’s finches. The method allows for the direct comparison between cDNA targets from two different species (each labeled with a different dye). The prevalence of one of the dyes for any of the genes on the resulting scan indicates a higher level of accumulation of transcripts from that gene in a particular beak morphology/species. The obtained expression profiles can be clustered to identify transcripts that are expressed in a species- and/or size-specific manner.


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Cold Spring Harb Protoc 2009: 119. [Abstract] [Full Text]

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This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
A. Abzhanov
Darwin's Finches: Analysis of Beak Morphological Changes During Evolution
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 Cold Spring Harb ProtocHome page
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Home page
 Cold Spring Harb ProtocHome page
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