Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5178

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Dellaporta Miniprep for Plant DNA Isolation

Detlef Weigel and Jane Glazebrook

Adapted from Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). CSHL Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This procedure provides a quick and inexpensive method for plant DNA isolation. When used with Arabidopsis, this procedure yields quite good-quality DNA that is suitable as a polymerase chain reaction (PCR) template for cleaved amplified polymorphic sequences (CAPS) or simple sequence length polymorphism (SSLP) markers. It yields enough DNA to test a large number of markers. It is recommended in cases when many markers will be tested on the same samples.


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J. D. Clarke
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Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5177 - pdb.prot5177.
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Home page
Cold Spring Harb ProtocHome page
D. Weigel and J. Glazebrook
Quick Miniprep for Plant DNA Isolation
Cold Spring Harb Protoc, March 1, 2009; 2009(3): pdb.prot5179 - pdb.prot5179.
[Abstract] [Full Text]