Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5191

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Similar articles in PubMed
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weisblat, D. A.
Right arrow Articles by Kuo, D.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weisblat, D. A.
Right arrow Articles by Kuo, D.-H.
Related Collections
Right arrow Laboratory Organisms, general
Right arrow Developmental Biology
Right arrow Emerging Model Organisms
Right arrowRelated Article
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

protocolProtocol

Devitellinization of Living Helobdella (Leech) Embryos

David A. Weisblat1 and Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

1Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Embryos of glossiphoniid leeches are enclosed in a thin vitelline envelope until "hatching" (stage 10). This protocol describes the procedure for removing the vitelline envelope from Helobdella robusta and H. sp. (Austin) embryos. This protocol is applicable to embryos of stages 1-9 but is probably more useful for early stages. With careful culturing, the devitellinized embryos can develop normally.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

Helobdella (Leech): A Model for Developmental Studies
David A. Weisblat and Dian-Han Kuo
Cold Spring Harb Protoc 2009: 121. [Abstract] [Full Text]

Related Protocol

Microinjection of Helobdella (Leech) Embryos
David A. Weisblat and Dian-Han Kuo
Cold Spring Harb Protoc 2009: 5190. [Abstract] [Full Text]



This article has been cited by other articles:


Home page
 Cold Spring Harb ProtocHome page
D. A. Weisblat and D.-H. Kuo
Helobdella (Leech): A Model for Developmental Studies
Cold Spring Harb Protoc, April 1, 2009; 2009(4): pdb.emo121 - pdb.emo121.
[Abstract] [Full Text]