Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5192

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Arnolds’ Silver Staining of Helbodella (Leech) Embryos

David A. Weisblat1 and Dian-Han Kuo

Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA

1Corresponding author (weisblat{at}berkeley.edu)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.


INTRODUCTION

Arnolds’ silver staining is used to visualize the boundaries of epidermal cells at the surface of embryos. As described in this article, silver staining can be performed on either living or fixed Helbodella (leech) embryos. To preserve the cell junction in fixed embryos, a special fixation procedure is used.


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Related Article

Helobdella (Leech): A Model for Developmental Studies
David A. Weisblat and Dian-Han Kuo
Cold Spring Harb Protoc 2009: 121. [Abstract] [Full Text]



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Home page
 Cold Spring Harb ProtocHome page
D. A. Weisblat and D.-H. Kuo
Helobdella (Leech): A Model for Developmental Studies
Cold Spring Harb Protoc, April 1, 2009; 2009(4): pdb.emo121 - pdb.emo121.
[Abstract] [Full Text]